Measurement

Part:BBa_K2447000:Design

Designed by: Chee Wai Kit (david)   Group: iGEM17_NUS_Singapore   (2017-10-03)


Extracellular phosphate sensor with GFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1162


Design Notes

Used a stronger RBS (RBS BBa_B0034) to elicit much stronger expression of GFP as compared to original system that was proposed by iGEM08_NYMU-Taipei which ultilised a weaker RBS (RBS BBa_B0032) for GFP expression (https://parts.igem.org/Part:BBa_K116404)


Source

From the iGEM parts registry

References