Coding
EGFP-tm86

Part:BBa_K2446051:Design

Designed by: Yijie Pan   Group: iGEM17_Fudan   (2017-10-21)


surface-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Surface-expressed EGFP (surEGFP) is built by fusing CD8α signal peptid and EGFP to the transmembrane region of CD86 (NM_175862) from N terminal to C terminal. CD8α peptide guides surEGFP to be located to cell membrane. transmembrane region of CD86 supports surEGFP to fix on membrane.

Source

From IDT （gblock).

References

[1] K. Newick, S. O'Brien, E. Moon, S. M. Albelda, CAR T Cell Therapy for Solid Tumors. Annual Review of Medicine 68, 139--152 (2017).