Composite
CMV-4-2

Part:BBa_K2446023:Design

Designed by: Yijie Pan   Group: iGEM17_Fudan   (2017-10-21)


CMV_4_PIP promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is aseembled by the specfic DNA binding sites and minCMV promoter through Biobrick assembly. We inset the DNA binding sites before the minCMV promoter. Then the DNA binding domain with VP64 can active the expression of this promoter. The activation intensity can be adjusted by change the repeats of DNA binding sites.

Source

Assembled by BBa_K2446053 (minCMV) and BBa_K2446011 4_PIP binding sites through Biobrick assembly.

References

[1] R. R. Beerli, D. J. Segal, B. Dreier, C. F. Barbas, Toward controlling gene expression at will: specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks. Proceedings of the National Academy of Sciences 95, 14628-14633 (1998).