DNA
4* RE2

Part:BBa_K2446011:Design

Designed by: Yijie Pan   Group: iGEM17_Fudan   (2017-10-21)


4_PIP binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used as a operator of our synthetic promoters. This operator will be inserted around the promoter of minCMV or Sv40 and bound by our synthetic transcription factor, which is made of PIP DNA binding domain fusing to VP64 or KRAB. In this way, we can create a group of activating or silencing synthetic promoter-transcription factor pairs in mammalian cells.

Source

This part was assembled with two BBa_K2446004 by Biobrick assembly in pSB1C3.

References

Fussenegger, M. et al. Streptogramin-based gene regulation systems for mammalian cells. Nature biotechnology 18, 1203--1208 (2000).