Regulatory

Part:BBa_K2442209

Designed by: Ailish O'Sullivan   Group: iGEM17_Glasgow   (2017-11-01)


mtlE wild type minimal promoter from P.fluorescens This is the native mtlE promoter from P.fluorescens. pmtlE is the intergenic region between mtlR and the mtlE coding region in Pseudomonas Flourescens

Usage and Biology

The mannitol operon exists naturally in P.fluorescens. The regulatory protein in this operon, mtlR, binds with sugar to induce the mtlE promoter. This promoter has found to only be inducible from the binding of the mtlR protein-sugar complex. This system naturally induces the transcription of mannitol metabolism genes within P.fluorescens.

This part was not submitted indivdually, it is part of our composite parts mtlE variant with B0032 mtlE with b0034 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None