Coding

Part:BBa_K2433002:Design

Designed by: Tamar Av-Shalom   Group: iGEM17_British_Columbia   (2017-10-25)


ietAS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 737
    Illegal BamHI site found at 1758
    Illegal XhoI site found at 659
    Illegal XhoI site found at 989
    Illegal XhoI site found at 3353
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2663
    Illegal NgoMIV site found at 3547
    Illegal AgeI site found at 3704
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 49
    Illegal BsaI.rc site found at 836
    Illegal BsaI.rc site found at 1838
    Illegal BsaI.rc site found at 3023
    Illegal BsaI.rc site found at 3305
    Illegal SapI.rc site found at 373


Design Notes

none


Source

Atu6082 and Atu6083 from pTiC58(https://www.ncbi.nlm.nih.gov/nuccore/159141737)

References