Composite

Part:BBa_K243012:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)

Strep-DigA-Middle Linker-Fok_i


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cloning steps were planned theoretically before we started the work in the wet lab. We used the middle linker because its length of 24bp is a bit shorter than the split linker we used. We had to find a compromise between connection stability and possible sterical interference between the single parts. The parts are fused according to RFC 25.

Commented GenBank file

Source

Combined the parts by serial cloning steps.

References