Coding

Part:BBa_K2429051:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-24)


pTRE 3 Exon mKate-HBG Reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2100
    Illegal XbaI site found at 30
    Illegal XbaI site found at 1505
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2100
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2100
    Illegal BamHI site found at 339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2100
    Illegal XbaI site found at 30
    Illegal XbaI site found at 1505
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2100
    Illegal XbaI site found at 30
    Illegal XbaI site found at 1505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 393


Design Notes

The second HBG intron has a point mutation at bp 654 to match the intron used in a paper that used the HBG intron as part of their reporter.



Source

The human beta globin introns came from the genome of HEK cells, and mKate from jellyfish.


References