Coding
Part:BBa_K2429042:Design
Designed by: Nia Myrie Group: iGEM17_MIT (2017-10-24)
pTRE L. shahii dCas13a-DDX6
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 6173
Illegal XbaI site found at 30
Illegal PstI site found at 2205
Illegal PstI site found at 2574
Illegal PstI site found at 3303 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 6173
Illegal PstI site found at 2205
Illegal PstI site found at 2574
Illegal PstI site found at 3303 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 6173
Illegal BglII site found at 949
Illegal BglII site found at 1573
Illegal BglII site found at 1969
Illegal BglII site found at 2260
Illegal BglII site found at 2350
Illegal BglII site found at 3511
Illegal BglII site found at 3598
Illegal BamHI site found at 339
Illegal BamHI site found at 4593
Illegal BamHI site found at 4695 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 6173
Illegal XbaI site found at 30
Illegal PstI site found at 2205
Illegal PstI site found at 2574
Illegal PstI site found at 3303 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 6173
Illegal XbaI site found at 30
Illegal PstI site found at 2205
Illegal PstI site found at 2574
Illegal PstI site found at 3303
Illegal NgoMIV site found at 4550
Illegal NgoMIV site found at 4569 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 935
Illegal SapI.rc site found at 1041
Illegal SapI.rc site found at 1911
Illegal SapI.rc site found at 2745
Illegal SapI.rc site found at 6051
Design Notes
Human Codon Optimized
Source
The catalytically active sequence of the protein came from L. shahii Cas13a on Addgene(https://www.addgene.org/79150/), which is also where our team received the original plasmid from. DDX6 seqeuence was taken from another plasmid received from another researcher in lab