Coding

Part:BBa_K2429037

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-24)


phEF1a rtTA3

This part is an expression vector that includes the coding sequence for rtTA3, a DOX-inducible transcription factor that regulates the Tet Response Element (TRE) promoter. This sequence lies downstream of the human elongation factor 1a (hEF1a) promoter, which is a low level constitutive promoter. Our team used other parts that make use of the TRE promoter so DOX induction in addition to this construct could be used to control the activity of the promoter. More specifically, the TRE promoter was used upstream of our RBP gene sequences to control and reporter constructs, and changed the amount of produced depending on the amount of DOX used applied to the system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 569
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None