Reporter
Part:BBa_K2429033:Design
Designed by: Nia Myrie Group: iGEM17_MIT (2017-10-24)
phEF1a mKate FF4
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1722
Illegal EcoRI site found at 2339
Illegal PstI site found at 315
Illegal PstI site found at 820 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1722
Illegal EcoRI site found at 2339
Illegal PstI site found at 315
Illegal PstI site found at 820 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1722
Illegal EcoRI site found at 2339
Illegal BglII site found at 569
Illegal BamHI site found at 1183
Illegal XhoI site found at 968
Illegal XhoI site found at 1619 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1722
Illegal EcoRI site found at 2339
Illegal PstI site found at 315
Illegal PstI site found at 820 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1722
Illegal EcoRI site found at 2339
Illegal PstI site found at 315
Illegal PstI site found at 820
Illegal NgoMIV site found at 703
Illegal AgeI site found at 81 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2244
Illegal SapI.rc site found at 1237
Design Notes
Ensure that the intron can be efficient spliced out of the construct.
Source
Part received from Weiss Lab