Composite

Part:BBa_K242900:Design

Designed by: Enrique Paz   Group: iGEM09_LCG-UNAM-Mexico   (2009-10-21)


[p(T3/T7)-GFP-(E3 + E9 toxins)]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 726


Design Notes

Until not expected, basal transcription of the toxins could be a problem. Those promoter recognized by T3 and T7 RNApol are not recognized by E.Coli -in theory-.


Source

composite

References