DNA

Part:BBa_K2429005:Design

Designed by: Adil Yusuf, Nia Myrie   Group: iGEM17_MIT   (2017-10-08)


pTRE 2-fluroescent protein (2-FP) reporter construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2620
    Illegal XbaI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2620
    Illegal BamHI site found at 339
    Illegal BamHI site found at 1750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2620
    Illegal XbaI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2620
    Illegal XbaI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since our other 2 exon report constructs used mHBG intron 2 and all of the targeting for our system is on mHBG intron 2, it is sequential before mHBG intron 1.


Source

Exons com from mutated versions of GFP from Aequorea victoria. Introns from human betaglobin gene from HEK293.

References