Part:BBa_K2424000:Design

As mentioned in the main page: one of the problems with NMT1 was that the original sequence contained an XbaI restriction site, making it incompatible with the RFC10-standard and thus not a viable part to use in an iGEM-project. Because of this, a nucleotide substitution was carried out at T796 in the sequence to remove said restriction site. This substitution was done after consulting with S. Pombe expert Sandra Lopez-Aviles from NCMM, to ensure that the risk it would cause a nonfunctional promoter would be minimal.

Documentation for the wildtype sequence before and after substitution can be found here: https://static.igem.org/mediawiki/2017/f/fe/T--UiOslo_norway--nmt1_before_after.txt

The sequence was obtained from an existing GFP expression system (pFA6a-kanMX6-nmt1-GFP) used in the laboratories at NCMM.