Regulatory

Part:BBa_K2424000

Designed by: Eirik Bager Sundmark   Group: iGEM17_UiOslo_Norway   (2017-08-23)


T796A NMT1 promoter

NMT1 promoter used in Schizosaccharomyces Pombe. The promoter is repressible by thiamine. It is the most commonly used promoter in for S. Pombe expression systems. The wildtype sequence of NMT1 contains an XbaI restriction site and is not RFC10-compatible. To get around this, a T->A nucleotide substitution was carried out at nucleotide 796 to remove the restriction site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
transcriptional
Parameters
None