Composite

Part:BBa_K2423003:Design

Designed by: iGEM Uppsala 2017   Group: iGEM17_Uppsala   (2017-08-18)


Zeaxanthin to crocetin enzymatic pathway


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1891
    Illegal NheI site found at 1914
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2079
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2486
    Illegal AgeI site found at 1774
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3003
    Illegal BsaI.rc site found at 672
    Illegal SapI.rc site found at 2111


Design Notes

We made complete devices including a promoter and RBS for each gene. We also included a 6x his tag for easy purification of the expressed protein.


Source

Crocus ancyrensis, Crocus sativus

References