Part:BBa_K242250:Design

antisense RNA targeting replication machineries of T3 and T7 bacteriophages

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The design included literature reading of examples to block the translation of proteins in bacteria, and particularly to those in which the target sequences were phages’ essential genes. The 5’ of the transcript should be blocked.
The secondary structures' free energies were all optimized (The less negative, the better) by recorring windows of different sizes within the target regions using the RNA folding server. The asRBSs are not blocked.

Source

bacteriophages T3 and T7 Genomes

References

We thank to Dr. Jose Luis Reyes Taboada for his assistance in the asRNA design.

Antisense mRNA-Mediated Bacteriophage Resistance in Lactococcus lactis subsp. lactis Appl Environ Microbiol. 1991 April; 57(4): 1109-1113
Essential Residues in the C Terminus of the Bacteriophage T7 Gene 2.5 Single-stranded DNA-binding Protein jbc June 28, 2006.
Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res. 31 (13), 3406-15, (2003)