Coding

Part:BBa_K242201:Design

Designed by: Jes�s Abraham Avelar Rivas   Group: iGEM09_LCG-UNAM-Mexico   (2009-10-11)

Colicin E9 DNase domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We took the sequence designed by Russell WALLIS et al. We included a RBS, eliminated preffix/suffix restriction sites and added two stop codons (taa).

Source

Escherichia coli strain J plasmid ColE9-J

References

Tandem overproduction and characterisation of the nuclease domain of colicin E9 and its cognate inhibitor protein Im9 Russell WALLIS, Ann REILLY, Karen BARNES, Chris ABELL,David G. CAMPBELL, Geoffrey R. MOORE, Richard JAMES and Colin KLEANTHOUS