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Part:BBa_K242051:Design

Designed by: Enrique Paz   Group: iGEM09_LCG-UNAM-Mexico   (2009-10-20)


Essential region of P4 bacteriophage genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2636
    Illegal NotI site found at 7087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 158
    Illegal BamHI site found at 171
    Illegal BamHI site found at 6566
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 343
    Illegal AgeI site found at 1378
    Illegal AgeI site found at 2200
    Illegal AgeI site found at 2494
    Illegal AgeI site found at 3433
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3512
    Illegal BsaI.rc site found at 5590


Design Notes

We remove all forbiden restriction sites, (EcoRI,XbaI,SpeI,PstI), from P4 genome but it means remove the integrase also. For our project it was useful because removing the integrase gene we expect P4 to be maintained as a plasmid. However it could mean a reduction in the stability of the plasmid without selection.



Source

This sequence comes from P4 bacteriophage genome from positions (0 to 18)+(4090 to 11624) http://www.ncbi.nlm.nih.gov/nuccore/9627507?report=graph&from=1&to=9087

References