Coding

Part:BBa_K2417008:Design

Designed by: Erica Stewart   Group: iGEM17_Sydney_Australia   (2017-10-25)


6x Poly-histidine Tag codon optimised for use in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to ensure efficient, consistent translation of the tag due to its importance in purification procedures, so we codon-optimised the sequence for use in Escherichia coli.


Source

N/A

References