Composite

Part:BBa_K2407314

Designed by: Xinyu Chen   Group: iGEM17_Tianjin   (2017-10-27)


TDH3 + vox + RFP + Adh1T + Ura3T + vox + β-carotene

Introduction

We use this composite to compare the phenomenon before and after mating. Initially, the Red Fluorescence Protein expresses. When the vika-vox system begin to work, the component between two vox sites will be deleted and the β-carotene gene will synthesize β-carotene. Yeasts turns from red to orange.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2653
    Illegal BglII site found at 4340
    Illegal XhoI site found at 2352
    Illegal XhoI site found at 2484
    Illegal XhoI site found at 3303
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2520
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5244
    Illegal BsaI site found at 7599
    Illegal BsaI.rc site found at 2080
    Illegal BsaI.rc site found at 7930
    Illegal SapI site found at 4644


[edit]
Categories
Parameters
None