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Other
Part:BBa_K2407104:Design
Designed by: Yan Zheng Group: iGEM17_Tianjin (2017-10-17)
Modified-Gal inducable promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 315
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence is modified for use in yeast or other AT-rich fungi. This part is a Modified-Gal inducable promoter working with the TF Z4EV. As a well functioned terminator, this part can perform well to control the expression of HO gene.
Source
It is obtained from Gene synthesis company after codon optimization
References
Houseley J1, Rubbi L, Grunstein M, Tollervey D, Vogelauer M. A ncRNA modulates histone modification and mRNA induction in the yeast GAL gene cluster.10.1016/j.molcel.2008.09.027.