![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K2407101:Design
Designed by: Yan Zheng Group: iGEM17_Tianjin (2017-10-17)
express the transcription factor, Z4EV
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 501
Illegal EcoRI site found at 1162
Illegal EcoRI site found at 1959
Illegal EcoRI site found at 1991
Illegal EcoRI site found at 2511
Illegal PstI site found at 1447
Illegal PstI site found at 1561 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 501
Illegal EcoRI site found at 1162
Illegal EcoRI site found at 1959
Illegal EcoRI site found at 1991
Illegal EcoRI site found at 2511
Illegal PstI site found at 1447
Illegal PstI site found at 1561 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 501
Illegal EcoRI site found at 1162
Illegal EcoRI site found at 1959
Illegal EcoRI site found at 1991
Illegal EcoRI site found at 2511
Illegal BglII site found at 1527
Illegal BglII site found at 1809 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 501
Illegal EcoRI site found at 1162
Illegal EcoRI site found at 1959
Illegal EcoRI site found at 1991
Illegal EcoRI site found at 2511
Illegal PstI site found at 1447
Illegal PstI site found at 1561 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 501
Illegal EcoRI site found at 1162
Illegal EcoRI site found at 1959
Illegal EcoRI site found at 1991
Illegal EcoRI site found at 2511
Illegal PstI site found at 1447
Illegal PstI site found at 1561 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1073
Illegal BsaI.rc site found at 840
Illegal BsaI.rc site found at 2566
Design Notes
This sequence is modified for use in yeast or other AT-rich fungi. This part is used to express the transcription factor, Z4EV. Z4EV are fast-acting and achieve a graded output response over a range of inducer concentrations. And the gene HO need to be promoted the transcription of Z4EV.
Source
We use overlap PCR to connect a piece of Can-A, TEF1 with Z4EV.
References
R. Scott McIsaac,* Benjamin L. Oakes, Xin Wang, Krysta A. Dummit, David Botstein, and Marcus B. Noyes Synthetic gene expression perturbation systems with rapid, tunable, single-gene specificity in yeast 10.1093/nar/gks1313