Measurement

Part:BBa_K2406071:Design

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-10-07)


Rox-Term-Slox Measurement Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 886
    Illegal AgeI site found at 998
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added overhangs to primers containing half of the target site/terminator sequence to create the final plasmid.


Source

We generated this part from round the horn PCR of part BBa_J04450

References