Part:BBa_K2406059:Design

Vlox-Term-Vlox Measurement Construct

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 888
  Illegal AgeI site found at 1000
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

PCR primers with overhangs were used to add the target sites and terminator in between promoter and RBS.

Source

This part was created from round-the-horn PCR of the BBa_J04450 part.