Regulatory

Part:BBa_K2398556

Designed by: Jan Mathony   Group: iGEM17_Heidelberg   (2017-10-27)


T7 Promoter

T7 promoter for protein Expression. The promoter was used by the iGEM Team Heidelberg in the context of phage assisted continous evolution (PACE).

Usage and Biology

Contribution: New documentation from Evry_Paris-Saclay 2020

This part is the strong promoter from T7 bacteriophage (taatacgactcactata) with GGG at 3' end.

The T7 RNA polymerase initiates the transcription at the first guanidine of this stretch of three G and it was shown that +1 GGG is one of the best +1, +2 and +3 base combinations at the transcription initiation for enhanced promoter strength [1].

References

[1] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None