Part:BBa_K2398029

OrganosiliconeRS-geneIII

We present the organosilicon-sensing riboswitch that regulates phage propagation by enhancing organosilicon-depending expression of geneIII that is vital for host cell infection.

Usage and Biology

The upstream riboswitch sequence can be adapted to any organosilicon of interest using the MAWS 2.0 software. So far, variants of this part have been used only for proof-of-principle applications but this part is designed to have a variety of applications in the cytochrome c engineering, for example, the phage-related discontinuous evolution (PREDCEL) approach.

Characterisation of the related part=

The results of the proof-of-concept of the related part are depicted in Fig. 1-3.

https://parts.igem.org/wiki/index.php?title=Part:BBa_K2398555

Figure 1: Used compounds for the characterization of the organosilicon-sensing riboswitch

Figure 2: Light emission detection of the NanoLuc reaction for different riboswitch activators and concentrations. Addition of compound (5) to the reaction resulted in increased enzyme activity as indicated by the two bars on the left-hand side compared to the compound (1) reaction.

Figure 3: Fold enzyme activity upon addition of compound (5) compared to compound (1) at either 5 mM concentration (left bar) or 15 mM concentration (right bar). At both concentrations, compound (5) exceeded the reaction activity of compound (1) by 1.5 fold.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1
  Illegal BglII site found at 1398
  Illegal BamHI site found at 733
  Illegal XhoI site found at 1384
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]