Coding

Part:BBa_K2398012:Design

Designed by: Moritz Przybilla   Group: iGEM17_Heidelberg   (2017-10-26)


LuxAB reporter for application in Phage-assisted continous evolution (PACE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BglII site found at 2304
    Illegal XhoI site found at 23
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 541
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1060


Design Notes

This was designed for the use with the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). It is meant for the usage as building block for accessory plasmids in the context of phage assisted continuous ecolution [1] or related systems [2].

The part is fully compatible with RFC10, as well as with Golden Gate assembly


Source

PCR amplified from plasmid

References

[1] Esvelt, Kevin M.; Carlson, Jacob C.; Liu, David R. (2011): A system for the continuous directed evolution of biomolecules. In: Nature 472 (7344), S. 499–503. DOI: 10.1038/nature09929.

[2]Brödel, Andreas K.; Jaramillo, Alfonso; Isalan, Mark (2016): Engineering orthogonal dual transcription factors for multi-input synthetic promoters. In: Nature communications 7, S. 13858. DOI: 10.1038/ncomms13858.