Part:BBa_K2398010
SD4-geneVI from M13 bacteriophage
This part provide bacteriophage M13 geneIII with a strong RBS, SD4[1], flanked by two homology regions for the usage due to the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). Figure one gives a short overview of our standard. Our BioBricks from the registry can easily be used for the assembly of blasmid with the standard (Fig.: 2).
This part was used in the context of a subproject of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/CRISPR).
Figure 3 gives a short overview on how the plasmid was used.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BglII site found at 455 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Rferences
[1] Ringquist, S.; Shinedling, S.; Barrick, D.; Green, L.; Binkley, J.; Stormo, G. D.; Gold, L. (1992): Translation initiation in Escherichia coli: sequences within the ribosome-binding site. In: Molecular microbiology 6 (9), S. 1219–1229.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BglII site found at 453 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |