Regulatory

Part:BBa_K2391000:Design

Designed by: Texia Loh   Group: iGEM17_Washington   (2017-10-26)


Isocitrate Lyase Gene Promoter (ICL1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Please note that ICL1 promoter is repressed under conditions where glucose is present. Therefore if biobrick is being used, please consider using other forms sugars, i.e galactose.

In order to characterize this promoter we had to think about what protein to use for expression and how to make a working plasmid for characterization.

Source

We had made sure that the sequence of interest had no restriction enzyme cut site that was similar to the prefix and suffix of the Biobrick Standard Assembly. Before ordering this sequence as a gBlock through IDT, a prefix and suffix was added. We have also designed a set of primers that is suitable to amplify the gBlock just in case a higher concentration of the linear sequence is needed to be used for the building of this biobrick.

References

FERNÁNDEZ, E., MORENO, F. and RODICIO, R. (1992), The ICL1 gene from Saccharomyces cerevisiae. European Journal of Biochemistry, 204: 983–990. doi:10.1111/j.1432-1033.1992.tb16720.x