Composite

Part:BBa_K2389040:Design

Designed by: Rochelin Dalangin   Group: iGEM17_UAlberta   (2017-10-26)


pT8O slightly lower activity


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 633
    Illegal BamHI site found at 1605
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 141
    Illegal NgoMIV site found at 551
    Illegal AgeI site found at 357
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Beyond the original difficulties with the first pT8O sequence, we just had to make sure our primers are compatible for a quikchange multi reaction.


Source

Bordetella pertussis

References