Composite

Part:BBa_K2389020

Designed by: Rochelin Dalangin   Group: iGEM17_UAlberta   (2017-10-26)


pT8O no detectable activity

This variant of the BACTH pT8O plasmid's leucine zippers have been mutated so that they no longer dimerize, thus resulting in no detectable activity of the adenylate cyclase

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 633
    Illegal BamHI site found at 1605
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 141
    Illegal NgoMIV site found at 551
    Illegal AgeI site found at 357
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


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