Translational_Unit

Part:BBa_K2387065:Design

Designed by: Bart Scholten   Group: iGEM17_Wageningen_UR   (2017-10-20)


eYFP[1-154]-nZIP and eYFP[155-238]-cZIP + araC/pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Long flexible linker to minimize steric hindrance.


Source

eYFP originates from the jellyfish Aequorea victoria

araC/pBAD is an endogenous E. coli promoter

All original parts are taken from the iGEM repository; [http://parts.igem.com/BBa_E0030 eYFP] [http://parts.igem.com/BBa_I0030 araC/pBAD promoter] [http://parts.igem.com/BBa_B0034 RBS BBa_B0034]

eYFPn[1-154] and eYFPc[155-238]was created in during [http://2017.igem.org/Team:Wageningen_UR Wageningen_URs 2017 iGEM project]. nLeucine Zipper and cLeucine Zipper are synthetic sequences ordered during [http://2017.igem.org/Team:Wageningen_UR Wageningen_URs 2017 iGEM project].


References