Composite
Part:BBa_K2387062:Design
Designed by: Bart Scholten Group: iGEM17_Wageningen_UR (2017-10-20)
eYFP[155-238]-cZIP + araC/pBAD promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
Long flexible linker to minimize steric hindrance.
Source
eYFP originates from the jellyfish Aequorea victoria
araC/pBAD is an endogenous E. coli promoter
All original parts are taken from the iGEM repository; [http://parts.igem.com/BBa_E0030 eYFP] [http://parts.igem.com/BBa_I0030 araC/pBAD promoter] [http://parts.igem.com/BBa_B0034 RBS BBa_B0034]
eYFPc[155-238] was created in during [http://2017.igem.org/Team:Wageningen_UR Wageningen_URs 2017 iGEM project]. cLeucine Zipper is a synthetic sequence ordered during [http://2017.igem.org/Team:Wageningen_UR Wageningen_URs 2017 iGEM project].