Composite
Part:BBa_K2387014:Design
Designed by: Stijn Prinsen Group: iGEM17_Wageningen_UR (2017-10-16)
Cpx system reporter + CpxP inhibitor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1199
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 636
Illegal AgeI site found at 748 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Strong tac promoter + strong RBS were chosen to increase CpxP production levels
Source
The mRFP1 under control of pCpxR originates from biobrick BBa_K339007 The CpxP coding sequence was isolated from genomic DNA of Escherichia coli K12