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Part:BBa_K2387004

Designed by: Bart Scholten   Group: iGEM17_Wageningen_UR   (2017-10-15)


eYFP N-terminus controlled by inducible araC/pBAD promoter

The N-terminus of fluorescent protein eYFP, N-terminal eYFP is put under the control of the L-arabinose inducible araC/pBAD promoter. Strong RBS BBa_B0034 is used to regulate transcription.

It is used as a control to test the absence of fluorescence of the sole N-terminal part of eYFP, which is used to test the functioning of the araC/pBAD promoter, and as a marker in BiFC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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