Generator
Part:BBa_K2381018
Designed by: Wenqi Huang Group: iGEM17_HZAU-China (2017-10-23)
light-activated rational split dCas9 with rigid linker
the light-activated split dCas9, which fused the split dCas9 with light-induced dimerization protein, can be activated under the irritation of blue light(470nm) BBa_K2381000 Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1420
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1420
Illegal NheI site found at 1179 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1420
Illegal BamHI site found at 4495 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1420
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1420
Illegal AgeI site found at 2357 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3155
[edit]
Categories
Parameters
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