Coding

Part:BBa_K2381009:Design

Designed by: Anqi Zhou   Group: iGEM17_HZAU-China   (2017-10-20)


Conventional split dCas9-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The split dCas9 is cut between the amino acid site 713 and 714. This part is the from site 714 to site 1368.

Source

The dCas9 is from Streptococcus pyogenes.

References

Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).