Part:BBa_K2381008

Conventional split dCas9-1

It is the first part of split dCas9 which is cut at the amino acid site 713 and 714. This part is the from site 1 to site 713.

References

Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1099
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]