Translational_Unit

Part:BBa_K2380043:Design

Designed by: Daniel Kelvin, Elena Nickels, Bea Marie Spiekermann, Lara Steinel, Stefan Zens, Tim Maier, Cristina Kurzknabe, Thomas Georg Wagner   Group: iGEM17_TU_Darmstadt   (2017-10-24)


Chitin deacetylase COD with T7 Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We needed a inducible Promotor for our project. (See our Wiki:http://2017.igem.org/Team:TU_Darmstadt/project/chitin_deacetylase)
The cod gene was fused to a prefixed T7-promoter (BBa_I719005) to provide high expression when T7 polymerase is present. In our special case, we use a split T7-RNA-polymerase to realize an orthogonal regulatory system.



Source

see basic parts

References