Coding

Part:BBa_K2380000:Design

Designed by: Lisa-Marie Brenner, Isabelle Feinauer, Werner Kleindienst, Cristina Kurzknabe, Isabelle Marquardt, Patrick Müller, Sven Storch   Group: iGEM17_TU_Darmstadt   (2017-10-14)


Chitin synthase NodC from Rhizobium leguminosarum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 373
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The enzyme was already expressed successfully in E.coli. [Kamst et al., 1995]. The sequence was taken from UniProt (A0A088MB42) and codon optimized for E. coli. The cleavage sites were investigated and adjusted for the BioBrick system.


Source

The gene is originating from the gram-negative bacteria Rhizobium leguminosarum. The sequence was taken from UniProt (A0A088MB42) and sequenced via IDT.

References