DNA

Part:BBa_K2377007:Design

Designed by: Zhicheng Lin   Group: iGEM17_Lanzhou   (2017-10-23)


EcrL, a developed element to generate Ecr hpRNA with loop120


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 368
    Illegal BglII site found at 895
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 856
    Illegal BsaI.rc site found at 407


Design Notes

Acyrthosiphon pisum ecdysone receptor (Ecr) is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003383721.1). ECR silencing can effectively reduce the expression of these key genes, thereby reducing the survival rate of insects and reproduction rate.

Source

arabidopsis thaliana

References