Coding

Part:BBa_K2375000:Design

Designed by: Sophie Vrba   Group: iGEM17_Minnesota   (2017-10-26)


PurA Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 388
    Illegal AgeI site found at 751
    Illegal AgeI site found at 1246
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1210


Design Notes

Illegal EcoRI site was removed and modified so it would not have an affect on the coding sequence.


Source

E. coli gDNA.

References