Coding

Part:BBa_K2371001:Design

Designed by: Qi Xiao   Group: iGEM17_BGIC-Union   (2017-10-22)


C-t7-dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1238
    Illegal BamHI site found at 973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2065
    Illegal NgoMIV site found at 3169
    Illegal NgoMIV site found at 3242
    Illegal NgoMIV site found at 3727
    Illegal NgoMIV site found at 4636
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Sequence of T7 polymerase is obtained from Eco.li BL21(DE3), whose genome is genetically modified to contain the coding sequence for T7 polymerase.

References

1.Yihao,Z.et al.,2017.Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains. ACS Synth. Biol., 2017, 6 (2), pp 211–216.

2.Tiyun,H.et al.,2016.Engineered photoactivatable genetic switches based on the bacterium phage T7 RNA polymerase.ACS Synthetic Biology,http://pubs.acs.org on October 31, 2016.