Part:BBa_K2368022

Introduction

Modified Gα Caenorhabditis elegans

Gα plays an important role in MAPK pathway. And in the yeast endogenous metabolic pathway, the conformation of Ste2 changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. In order to make the sweetness receptor fit the Gα better, we modified the Gα. Then the sweetness receptor can combine with the Gα and send the signal to the downstream.

Fig.1 The structure of Gα in Caenorhabditis elegans

Design

Mutated C-terminus 15bp sequence of the yeast Gα, replace it with the Caenorhabditis elegans Gα. After construct the modified Gα by PCR, and the initial sequence is amplified from Saccharomyces Cerevisiae CEN.PK2-1C..

Fig.2 The schematic diagram of Modified Gα- Caenorhabditis elegans. Line 2 is the result of Gα- Caenorhabditis elegans

Experiment

The part is used to improve the matching condition of sweetness receptor and the modified Gα. The length of sequence is 1418bp.

Fig.3 Electrophoresis of the positive result of the modified Gα- Caenorhabditis elegans

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]