RNA
Part:BBa_K2361003:Design
Designed by: Thijs Ettema Group: iGEM17_Groningen (2017-10-10)
pUSP45 tracrRNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is essential for a functional CRISPR system, as the crRNA:tracrRNA;dCas9 complex is needed for signalling.
Source
The promoter sequence, together with the tracr RNA was obtained by synthesis based on designs from the team.