Device

Part:BBa_K2360014:Design

Designed by: CHEN NANZHU   Group: iGEM17_SCUT-China_A   (2017-10-27)


rrnB' terminater+CadR+pCadA+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1453


Design Notes

We use the standard biobrick to make this part.


Source

We combined the GFP module (BBa_E0240) and cadmium detecting module(BBa_K2360005) to make this part.

References

[1]. Brocklehurst K R, Megit S J, Morby A P. Characterisation of CadR from Pseudomonas aeruginosa: a Cd(II)-responsive MerR homologue[J]. Biochemical & Biophysical Research Communications, 2003, 308(2):234-9.
[2]. Leveau J H, Lindow S E. Predictive and interpretive simulation of green fluorescent protein expression in reporter bacteria[J]. Journal of Bacteriology, 2001, 183(23):6752.