Device

Part:BBa_K2360009:Design

Designed by: CHEN NANZHU   Group: iGEM17_SCUT-China_A   (2017-10-27)


rrnb' terminater+RcnR+pRcnR +SRRz+rrnb terminater


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 296
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use the standard biobrick to make this part.


Source

We combind the lysis module (BBa_K2360000) and nickel detecting module(BBa_K2360004) to make this part.

References

[1]. Iwig J, Rowe J, Chivers P. Nickel homeostasis in Escherichia coli - the rcnR-rcnA efflux pathway and its linkage to NikR function[J]. Molecular Microbiology, 2006, 62(1):252.
[2]. Kloos D U, Strätz M, Güttler A, et al. Inducible cell lysis system for the study of natural transformation and environmental fate of DNA released by cell death.[J]. 1994, 176(23):7352-7361.