Composite

Part:BBa_K2360004:Design

Designed by: HU RIRONG   Group: iGEM17_SCUT-China_A   (2017-10-27)


rrnb' terminater+RcnR+pRcnR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 296
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We combinded with the rrnb' terminator for a convenient using.


Source

We synthesized this part, optimized for E.coil K12.

References

[1]. Iwig J, Rowe J, Chivers P. Nickel homeostasis in Escherichia coli - the rcnR-rcnA efflux pathway and its linkage to NikR function[J]. Molecular Microbiology, 2006, 62(1):252.