Part:BBa_K2360004

rrnB'terminator+RcnR+pRcnR

This part is an operon which can make a specific response to nickel ion. We add a strong terminator in 5', so that we can just add a report module in 3' to make a biosensor. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Figure 1. Bio-circuit of BBa_K2360004

Lysis gene expression

We have developed the lysis module (BBa_K2360000) and five metal detecting module for E. coli BL21. Combining the lysis module and detecting module, we have constructed five devices which can respond to different metal ions. Because the lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of our engineering bacteria in different concentration of metal ions to prove its function.

Figure 1. the growth curve of nickel detecting device in different concentration of nickel ions.
we measured the growth curve of our engineering bacteria in different concentration of nickel ions.It was a pity that we found the OD₆₀₀ of E. coli with did not decline after we added Ni²⁺, which showed this devices did not work as we expected.